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Thursday, October 22, 2020 | History

2 edition of biological assay of strophanthus (kombé) in comparison with ouabain found in the catalog.

biological assay of strophanthus (kombé) in comparison with ouabain

J. H. Gaddum

biological assay of strophanthus (kombé) in comparison with ouabain

by J. H. Gaddum

  • 282 Want to read
  • 39 Currently reading

Published by Pharmaceutical Press in London .
Written in English

    Subjects:
  • Biological assay.,
  • Strophanthus kombe.

  • Edition Notes

    Cover title.

    Statementby J. H. Gaddum.
    The Physical Object
    Paginationp. [274]-300 :
    Number of Pages300
    ID Numbers
    Open LibraryOL18633876M

    Overview Information Strophanthus is an herb. It has been used as an arrow poison in Africa. Strophanthus seeds are used to make medicine. Despite serious safety concerns, people take strophanthus. A. Biological Conversion Studies B. Distribution and Excretion Studies C. Synthesis of SLabeled Compounds D. Protein Turnover in Vivo and in Vitro E. Reversibility of Cysteine and Cystine Decomposition Chapter XII. Alkali Metal and Alkaline Earth Tracers 1. General Survey of Alkali Metal Tracers 2. Preparation, Properties, and Assay.

    First Supplement to USP 35–NF 30 General Information / 〈〉 Biological Assays Process Characterization cell culture reagent such as fetal bovine serum. The fitness for use in such cases is tied to the ability of the assay to Bioassays may be performed to assess the effect on drug screen reagent lots and to ensure that lots that may bias or.   Strophanthus is an herb. It has been used as an arrow poison in Africa. Strophanthus seeds are used to make medicine. Despite serious safety concerns, people take strophanthus for “hardening of the arteries” (arteriosclerosis), heart problems, and high blood pressure.

    Additional Physical Format: Online version: Emmens, C.W. (Clifford Walter). Principles of biological assay. London, Chapman & Hall, (OCoLC) Ready-to-Use Cell-Based Assays for Comparability and QC Lot Release Testing Pharmaceutical companies are developing an increasing number of biologics, biosimilars and biobetters, thereby creating a need for MOA-reflective cell-based assays that can support multiple stages of the biopharmaceutical development pipeline.


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Biological assay of strophanthus (kombé) in comparison with ouabain by J. H. Gaddum Download PDF EPUB FB2

An illustration of an open book. Books. An illustration of two cells of a film strip. Video. An illustration of an audio speaker. Audio. An illustration of a " floppy disk. The standard for the biological assay of strophanthus Item Preview remove-circle Share or Embed This : Purpose of biological assay Biological assays are methods for the estimation of nature, constitution, or potency of a material (or of a process) by means of the reaction that follows its application to living matter.

(I) (II) Fiducial limits for the strophanthus assay. Ratio of individual extracts in co-extract mixture. A 3-to-2 weight/weight ratio of Strophanthus hispidus (roots) to Aframomum meleguta (seeds) derived from the best ethnomedicinal practices and guidelines was utilized for all concentrations of the co-extract in all assays (anti-inflammation, anti-microbial and anti-oxidant) as stated in Materials and : John Kenneth Mensah, Amina Ibrahim, Yakubu Jibira.

Since my book was published, in which I first mentioned this “insulin of the heart”, we have, at my clinic, started a program to make strophanthus extract available to all heart patients who wish to use it – (note from Ben: I found that it can also be found as Strophanthus on Amazon, but cannot vouch for the quality).

Its aqueous solution is precipitated by lead acetate. To assay tincture of strophanthus for strophanthin, Mr. Barclay proposes to convert this active principle by hydrolysis into the more positive strophanthidin. (See two interesting papers on this subject in Pharm.

Jour. Strophanthus consists of the dried seeds of the seeds of Strophanthus kombe Oliver, freed from the awns. The plants are a climber and belong to the family Apocynaceae. Geographical Source: The plant is indigenous to central East Africa, which serves principal commercial source of the drugs.

Strophanthus is a genus of flowering plants in the family Apocynaceae, first described as a genus in It is native primarily to tropical Africa, extending to South Africa, with a few species in Asia from southern India to New Guinea and southern China. The name (strophos anthos, "twisted flower") derives from the long, twisted, threadlike segments of the corolla, which attain a length of.

Coronary artery disease is currently the leading cause of death in the United States. Despite the increasing sophistication of surgical techniques, the introduction of new techniques such as balloon angioplasty, and a number of new drugs (e.g. beta blockers, calcium antagonists), it is estimated that over 1 million heart attacks will occur this year, resulting indeaths.

Gaddum was a member of the sub-committees on the biological standards for digitalis, strophanthus and ergot for the British Pharmacopoeia, he also served on the subcommittee dealing with the.

This book presents scientific documentation of the correlation between the observed folk use and demonstrable biological activity, as well as the characterized constituents of the plants.

Year: Edition: 2. Publisher: CRC Press. Language: english. Pages: / ISBN E.J. Umberger, G.H. Gass, J.M. Curtis, Design of a biological assay method for the detection and estimation of estrogenic residues in the edible tissues of domestic animals treated with estrogens Endocrinology () Methods of Biological Assay Depending on a Quantal Response,†Special Report ; Med.

Res. Council (). 11 Raymond-Hamet, Determination de la Dose de Digitaline Cristallisee Nativelle qui est Lethale pour la Grenouile et Application a Cette Determination de la Courbe de Toxicite Dite de Trevan Rev. Pharmacologie et therap. exp. 4: ( Kawaguchi K., Hirotani M., Furuya T. () Strophanthus Species (Members of the Dogbane Family): In Vitro Culture and the Production of Cardenolides.

In: Bajaj Y.P.S. (eds) Medicinal and Aromatic Plants IV. Biotechnology in Agriculture and Forestry, vol DescriptionThe name Strophanthus is derived from the Greek strophos (a twisted cord or rope) and anthos (a flower), thus expressing the chief peculiarity of its appearance, the limb of the corolla being divided into five, long, tail-like official description of the seeds is 'lance-ovoid, flattened and obtusely-edged; from 7 to 20 mm.

in length, about 4 mm. in breadth, and. Strophanthus is a muscle poison; it increases the contractile power of all striped muscles. Acts on the heart, Increasing the systole and diminishes the rapidity. May be used with advantage to tone the heart, and run off dropsical accumulations.

BSPBS Publications Agriculture and Allied Sciences 2 BSP Books Pvt. Ltd. Phone:Fax: 91+ e-mail: [email protected] Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis 3 rd Ed.

Harborne A.J. Rpt. Results: The study was fruitful by the effect of Strophanthus hispidus on infarction size, which got reduced to ± and ± by mg/Kg and mg/Kg ethanolic extracts which was remarkably significant when compared with that of the control group ± The plant extract did reduce heart rate at various time intervals.

There was also a protective effect in the case of. Strophanthus is an herb. It has been used as an arrow poison in Africa. Strophanthus seeds are used to make medicine. Despite serious safety concerns, people take strophanthus for “hardening of the arteries” (arteriosclerosis), heart problems, and high blood pressure.

Methods: The antioxidant and AChE-inhibitory properties of the methanol extracts of Strophanthus preussii were evaluated by standard in vitro methods viz DPPH (2,2-diphenylpicrylhydrazine), nitric oxide (NO), hydroxyl radical (OH) and hydrogen peroxide (H 2 O 2) radical scavenging assays as well as reducing power, iron (II) [Fe 2+]/ascorbate-induced lipid peroxidation (LPO) and AChE.

A bioassay is an analytical method to determine concentration or potency of a substance by its effect on living cells or tissues. Bioassays are quantitative biological assays used to estimate the potency of agents by observing their effects on living animals (in vivo) or tissue/cell culture systems (in vitro).

A bioassay experiment can either be qualitative or quantitative, direct or indirect. ABSTRACT: Antibacterial and antioxidant activities of Strophanthus hispidus stem aqueous and organic (cyclohexane, chloroform, ethyl acetate and ethanol) extracts were investigated in order to assess its use in traditional vitro antibacterial activity assessed by Disc diffusion and Microdilution methods, was tested against Gram positive bacteria (sensible Staphylococcus aureus) and.Ouabain is a natural substance that occurs in African plants.

Until the s, it was used in Europe and especially in Germany for the treatment of heart failure. Ouabain has polarized the medical profession like hardly any other drug.

Supporters celebrated Ouabain as insulin of the heart, opponents denigrated this drug as placebo. Euphoric praise and devastating criticism characterised an. OR Detection and measurement of the concentration of the substance in a preparation using biological methods.

3. Bioassays, as compared to other methods of assay (e.g. chemical or physical assay) is very important. Because it is the only method of assay if 1.